It has been two years since the Tendoshuu invaded the O-Edo Central Terminal. Since then, the Yorozuya have gone their separate ways. However, Gintoki Sakata anticipates Utsuro's return and begins searching Earth's ley lines for traces of his Altana. After encountering remnants of the Tendoshuu who continue their quest for immortality, Gintoki returns to Edo. Later, the reunited Shinsengumi and Yorozuya launch an attack on the occupied Central Terminal. With the Altana at risk of detonating from the wreckage of the Tendoshuu's ship, the Yorozuya and their allies fight their enemies while safeguarding Edo and the rest of the world. In accordance with their teacher's wishes, Shouyou Yoshida's former students unite one last time to relive their pasts in an attempt to secure their futures.
As of March 2021, Gintama: The Final has earned a franchise record of 1.85 billion yen (16.94 million USD) and debuted at No. 1 at the Japanese box office. The film concludes the Gintama anime series, adapting chapters 699-704 of the original manga with new story elements.