Yotsuba is a character known for being lively, joyful, and inquisitive. With great enthusiasm, she seeks to uncover all of her brothers' secrets. She carries around a magnifying glass as part of her investigative tools. When she discovers something intriguing, she says "checki". Yotsuba is from England and was introduced in 2000 through Dengeki Gs magazine and the first Sister Princess game. In Sister Princess RePure, she raises an interesting question about how much they all truly know about their brother. Along with Rinrin, she embarks on a mission to learn more about him and deepen her bond with him.