The Matsuno family consists of six playful and rebellious sons (who are sextuplets), headed by the oldest brother Osomatsu. Although they were students during the Showa era, their home and lifestyle have been greatly influenced by contemporary society's advancements in technology and urbanization.
Osomatsu-san is based on Studio Pierrots original TV anime series of the same title, which itself is a spin-off of Fujio Akatsukas manga series Osomatsu-kun.