In 2005, Ayumi Tsuji joined the talent agency Pro-Fit after completing its training school in Tokyo following high school graduation. She made her debut as an extra character in Boukyaku no Senritsu in 2004. Later on, she left Pro-Fit in 2011 and became a freelance seiyuu for about one and a half years before joining Mausu Promotion in September 2012. On July 7, 2017, it was announced that Tsuji would marry fellow seiyuu Michiru Satou.