Ono Daisuke was previously affiliated with Aoni Production through its voice acting school and Mausu Promotion as his agency since the start of his career in 2001. As of February 1, 2016, he became a freelance. He hosted MAG Net between April 2014 to March 2013 with fellow seiyuu Saori Gotou. Together with Kamiya Hiroshi, he is the host of 神谷浩史・小野大輔のDear Girl ~Stories~ which is one of the oldest and most popular seiyuu-hosted radio programs. He has hosted various other programs including Ono Daisuke, Kondou Takayuki no Yume Bouken~Dragon&Tiger with seiyuu Takayuki Kondou.
In addition to his work as a voice actor and host, Ono Daisuke formed the air band "MASOCHISTIC ONO BAND" with Kamiya Hiroshi that debuted at Nippon Budoukan in 2013. He is also part of the singing sub unit of "DABA," a project created in 2008 bringing together Fukuyama Jun, Hino Satoshi, Tachibana Shinnosuke, Suganuma Hisayoshi, Majima Junji and Kondo Takayuki. He is also the leader of "CABA," an active singing sub unit since 2014.
Ono Daisuke is the leader and host of the annual OrePara live (founded in 2008), with Suzumura Kenichi, Morikubo Showtaro & Terashima Takuma (replacing Iwata Mitsuo from 2014 onward). In DGS radio program, he has admitted that he is not fond of cats because of his unpleasant childhood experience with them. However, it seems that Ono Daisuke has no problem at all with Nyanko-sensei or Nya-san, a cat that belongs to his fellow seiyuu Kamiya Hiroshi.
He dislikes raisins but loves Chinese noodles, nuts and almonds. He also has a certain fascination in Buddha statue. The first time he saw the biggest Buddha statue in the world was on his birthday celebration in Ushiku-shi, a place he had wanted to visit, which is also Kamiya Hiroshis hometown.
Ono Daisuke has received several awards for his work as a seiyuu, including Best Supporting Actor at the 2nd Seiyuu Awards (2008), Best Actor at the 4th Seiyuu Awards (2010) and Best Actor & Best Personality at Tokyo Anime Awards (2015). He has also been recognized as the Best Voice Actor.