Ai Kayano discovered her passion for voice acting while watching foreign films that were dubbed into Japanese. Although she learned about the seiyuu profession during high school, initially, she was interested in pursuing a career as a beautician after completing beauty vocational school. However, she later enrolled at Pro-Fit Voice Actors School and graduated in 2010, marking her debut as a professional seiyuu that April. Kayano began with minor roles before landing her first regular role as Itsuwa in Toaru Majutsu no Index series. In 2011, she starred in Ano Hi Mita Hana no Namae wo Bokutachi wa Mada Shiranai as Meiko Honma, which earned her the title of Newcomer of the Year at the 6th Annual Seiyuu Awards. Subsequently, Kayano left Pro-Fit talent agency and joined Office Osawa in January 2015. Aside from her work as a seiyuu, Kayano is also known for hosting a web program called Kayanomi where she samples different types of sake.